Hieff clone enzyme
WebBest practices. With focuses on the research and development and manufacturing of tool enzyme raw materials, antigen and antibodies, Yeasen supplies 40+ types of enzymes, … Web6 de jun. de 2024 · Restriction enzymes were purchased from New England BioLabs. Hieff Clone™ Plus One Step Cloning Kit was purchased from Shanghai Yeasen Biotech. All the primers used in this study were listed in Table S5. 3.3 Growth Condition of …
Hieff clone enzyme
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Web655.00. 产品描述. Hieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒，精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反应所需缓冲液，并添加了独特的重组增强因子，可显著提高重组克隆效率。. 该试剂盒可以将 PCR产 … Web10912-A 2× Hieff Clone® MultiS Enzyme Premix 50 μL 100 μL 10912-B 500 bp Control Insert (25 ng/μL) 5 μL 5 μL 10912-C pUC 19 Control Vector, linearized (50 ng/μL) 5 μL 5 …
WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 … Web1 de nov. de 2024 · Enzymes immobilized on solid supports have been certified to improve biocatalytic reusability. Hence, the immobilization of the polymers on solid ... amplified products were inserted into the recombinant plasmids pET22b-Linker1-SpyTag and pET22b-Linker2-SpyCatcher by Hieff Clone Plus One Step Cloning Kit (Yeasen Biotech Co., Ltd ...
WebCompared with the traditional enzyme ligation technology, the seamless cloning kit is simple and fast to operate, and is not limited by the restriction of the enzyme cleavage site. It can splicing multiple fragments at one time, which greatly improves the cloning efficiency. This Hieff Clone™ universal one step cloning kit is available for 1 ... WebHere's a list of top tips to keep in mind when designing your primers for seamless cloning, including some information specific to In-Fusion Cloning. The 3′ end of the primer should contain sequence that is specific to the target gene you are amplifying. This sequence should be 18–25 bases long and should ideally have a GC content between ...
Web2× Plus PCR Master Mix (With Dye) is a ready-to-use 2x premixed solution containing Hieff Canace™ Plus high-fidelity DNA polymerase, dNTPs, and an optimized buffer. This PCR …
http://www.labbase.net/Supply/SupplyItems-4428942.html ea account anlegenWeb27 de jun. de 2024 · A final extension step at 68 °C for 10 min was conducted at the end of the PCR reaction. 2 μL DpnI enzyme was then added to the PCR reaction mixture to digest the template plasmid pET28a–bFMO at 37 °C for 1 h; DpnI-digested PCR products were finally self-ligated by Hieff clone Enzyme Premix. ea access vs ea playWebFor GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine … ea account bewerkenWebHieff Clone® Plus One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任何载体的任何位点，可高效克隆50 bp-10 kb片段。将 … ea account beveiligenWebOnce the Entry clone is ready, the gene of interest is easily shuttled to a secondary plasmid, the Destination vector. This reaction is mediated by a robust enzyme mixture called LR Clonase™, which contains the necessary protein activity to excise the gene of interest from the Entry clone and integrate it into the Destination vector, which then becomes your … csgo in chinaWeb25 de nov. de 2024 · Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products. Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult because of the many genes involved in central metabolism. In previous studies, … csgo incendiary grenadeWebHIF-1 alpha cDNA clone Background. All these HIF-1 alpha cDNA clone are full sequence confirmed. There are 15 HIF-1 alpha expression cDNA clones with various fusion tags, … csgo increase fov